Upcoming Courses for BiacoreT100:

title Check Courses.


Biacore T200:
arrow User's Guideline
arrow Training Checklist
arrow Simplified Protocol
arrow SCK Protocol

Biacore 8K:
arrow 8K sample submission guideline

BCF Access :

Reservation Procedure



Biacore Simulation Tool: "Biacore Simul8"
Link here!

BCF invited Seminar:

2020 Biacore Workshop open lectures: Small Molecules- Working with DMSO.
Time: Oct. 20 (Tue).
Speaker: Sindy Li, Cytiva
Registration Here!

Starting NOW to the end of 2018, 50% off on your lab's first submission to Biacore 8K (Sinica users only). Please submit your samples through BCF reservation system.

Biacore Webinar:

Recent Upgrade:

Please refer to the T100 2.1.0 control software release notes for details.

BCF stock:

* Amine Coupling Kit
* 7mm tubes
* Desorb solutions

Available chips for testing nonspecific binding:

* C1 chip
* CM3 chip
* CM5 chip
* SA chip
* NTA chip

Capture kits (Ask before plan your experiments!):

* His capture kit
* Human antibody capture kit
* Mouse antibody capture kit
* GST capture kit



GE Biacore 8K, T200 and T100

Biacore T100 Biacore system is a series of Surface Plasmon Resonance (SPR) instruments. The instrument contains a flow system, a temperature control unit and an optical system to measure the molecular interactions occuring on sensor chips in real-time, with/without special labeling. This technique is capable for binding kinetics (on/off rate constants), affinity (equilibrium constants)and thermodynamic studies.

Important Notification:

* (2020-12-23) Both Biacore T200 and Biacore 8K are ready.

* (2020-12-11) Biacore T200 is not available due to an unknown injection error. Service is called.

* (2020-12-01) Sample submission to Biacore 8K is suspended. Sorry for the inconvenience.

* (2019-03-01) Biacore 8K is ready for services. Please refer to 8K Sample submission guideline for sample requirements.

* (2017-09-06) The temperature control units on both Baicore T200 and T100 are getting old and the cooling rate is not within instrument specification. Temperature below 25℃ is not recommended. Longer waiting time might be required for temperature to stabilize. Please check with Dr. Jao before planning your experiments.

* Please use sealing foils with no glue applied inside of the wells. Otherwise, the needle can get clogged and will not deliver samples correctly.

title Selected Applications

Binding specificity
Binding kinetics
Active protein concentration
Molecular Recognition
Ligand fishing
Structure-function Relationship

title Specification

Spec. Biacore T200 Biacore 8K
No. of flow cells
Flow rate
1 - 100 μl/min
1 - 100 μl/min
Injection volume
2 - 350 μl
1 - 200 μl
Required volume
injection volume + 20 - 50 μl
injection volume + 20 - 50 μl
Flow cell volume
0.06 μl
0.04 μl
Sample/reagent capacity
1x96/384-well plate+33 vials
4x96/384-well plates
Temperature range
10* to 45 ℃ (*BCF instruments)
(Room temp. -20) to 40 ℃
Refractive index range
1.33 - 1.39
1.33 - 1.39
Baseline noise
<0.03 RU
<0.02 RU
1E3 - 3E9/Ms for proteins
1E3 - 5E7/Ms for small molecule
1E9/Ms for proteins
1E3 - 5E7/Ms for small molecule
1E-5 - 1 /s
1E-6 - 0.5 /s
Sample concentration
Minimum molecular weight


title Suggested Reading:

Biacore T200 Instrument Handbook.

Biacore T200 Software Handbook.

Rich RL and Myszka DG, 2008, Survey of the year 2007 commercial optical biosensor literature, J. Mol. Recognit., 21, 355-400.

Rich RL and Myszka DG, 2007, Survey of the year 2006 commercial optical biosensor literature, J. Mol. Recognit., 20, 300-366.

Schasfoort RBM and Tudos AJ (eds.), 2007, Handbook of Surface Plasmon Resonance. RSC Publishing.

Rich RL and Myszka DG, 2006, Survey of the year 2005 commercial optical biosensor literature, J. Mol. Recognit., 19, 478-534.

Single-Cycle Kinetics:
Karlsson R, Katsamba PS, Nordin H, Pol E and Myszka DG, 2006, Analyzing a kinetics titration series using affinity biosensors, Anal. Biochem., 349, 136-147.

Fragment Based Screening / Small Molecules:
Anthony M. Giannetti, 2011, From experimental design to validated hits, Methods in Enzymology, 493, 169-218.