Octet Red96

Menu:

Invited Seminars:

2017 BLI Octet RED96 Antibody Workshop:
Time: Nov. 8 (Wed.)
Place: IBC 409
Speaker:
Mr. Hendrick Loei, Pall
Registration now!
Workshop poster Here.

* SA sensor
* SSA sensor
* AR2G sensor
* NTA sensor
* HIS 1K sensor

 

title Introduction

Octet RedThe Octet Red96 system is a biolayer interferometry biosensor for measuring protein concentration, the kinetics and affinity of intermolecular interactions. With the 96-welled microplate design, it is applicable for screening of protein-protein and protein-small molecules interaction.

For more detail, please visit Ocetet Red96 website.

Important Notification:

* (2017-06-21) The Data Acqusition and Data Analysis softwares are both upgraded to version 10.0. Users can still access version 7.0 on the desktop.

* (2016-08-15) Octet RED96 is down because the shaker made big noises. Service has been called. Sorry for the inconvenience.

* (2016-03-21) Octet RED96 is back for service. The system was not stable because of possible virus infection. Please note that USB drives are always forbidden on the control PC!

* (2016-03-18) Octet RED96 is down because of control PC problems. Service has been called. Sorry for the inconvenience.

* (2015-05-14) Octet RED96 is back for service. Please leave the lamp on when you are done with your experiments.

* (2015-04-30) Octet RED96 is down because of lamp problems. Service has been called. Sorry for the inconvenience.

title Specification

Sample volume
180 - 220 μl
Orbital Flow Capacity
100 - 1500 rpm
Automation
8 biosensors in parallel
Sample/reagent capacity
1x96-well plate
Temperature range
(Ambient + 4℃)–40 ℃
Baseline noise
<=3 pm
ka
1E1 - 1E7/Ms for proteins
1E3 - 1E6/Ms for small molecules
kd
1E-6 - 0.1 s for proteins
1E-3 - 1 s for small molecules
Range for hIgG
0.5~2000 µg/mL @200rpm
0.05~100 µg/mL @1000rpm
Minimum molecular weight for kinetics
>150Da

For more detail, please refer to Octet System Data Acquisition Software User Guide.

title Suggested Reading:

Octet System Data Acquisition Software User Guide

Octet Data Analysis Software 7.0 User Guide

Concepcion J. 2009 Label-free detection of biomolecular interactions using BioLayer interferometry for kinetic characterization. Comb Chem High Throughput Screen., 12, 791-800.

Ekiert et al., 2011 A Highly Conserved Neutralizing Epitope on Group 2 Influenza A Viruses, Science, 333, 843-850.

Martin et al., 2011, Novartis Evaluation of the ForteBio Octet RED: A Versatile Instrument for Direct Binding Experiments. In Label-Free Technologies for Drug Discovery, Matthew Cooper and Lorenz M. Mayr (eds). John Wiley & Sons Ltd.; pp.224-240.

Wartchow CA. 2011 Biosensor-based small molecule fragment screening with biolayer interferometry. J Comput Aided Mol Des. 25, 669-676.

Chris M. Maragos 2011 Detection of deoxynivalenol using biolayer interferometry Mycotoxin Res. 27, 157-165.