Chirascan-plus qCD spectrometer
The Chirascan-plus qCD is located in IBMS N133, Dr. Chinpan Chen's laboratory. This instrument measures circular dichroism and absorbance spectra simultaneously. In addition to CDNN, a protein secondary structure prediction program, two extra analysis softwares, Global 3 Thermodynamic Analysis and qBIC Biocomparability Suite, provide dynamic multimode (CD, absorbance, temperature) analysis and statistical comparison of sets of spectra at far-UV and near-UV regions.
Please refer to Instrument Manual for details.
Important Notification:
* (2018-06-13) Chirascan+ is available for service.
* (2018-04-16) Chirascan+ is not available because of lamp problems. Service is called. Sorry for the inconvenience.
* (2017-04-03) Free qCD sample tests available for Sinica users. Please apply sample submission through BCF reservation system.
Specification
| Wavelength range | 165 nm to 1200 nm |
| Baseline drift | <= 0.01 mdeg/℃ after warm-up |
| Temperature range | 0 - 100℃ |
| Cuvatte | QS Quartz 0.1, 0.5, 1, 10 mm |
Suggested Reading:
Kelly SM, Jess TJ, Price NC, 2005, How to study proteins by circular dichroism, Biochimica et Biophysica Acta, 1751:119-139.
Analysis of circular dichroism data, 2004, Methods Enzymol., 383:282-317.
Determining the CD Spectrum of a Protein", Currents Protocols in Protein Science, 2004, 7.6.1-7.6.24, John Wiley & Sons, Inc.
Softwares for Protein Secondary Structure Analysis
CDNN by Dr. Gerald Böhm, Berlin
DichroWeb by Birkbeck College, Unversity of London, UK
CDPro by Colorado State University, Fort Collins, Colorado, USA DichroProt by Institut de Biologie et Chimie des Protéines, Lyon cedex, France